PEPTIDE HANDLING & PREPARATION GUIDE

RESEARCH PEPTIDE HANDLING & PREPARATION GUIDE

Educational & Laboratory Reference Only

The information below is provided strictly for educational and research reference purposes. All compounds listed are supplied as research materials only, for in-vitro and laboratory use. This guide reflects commonly cited laboratory handling conventions and mechanistic discussions found in published research literature. It does not constitute medical advice, treatment guidance, or instructions for use in humans or animals, and no amount, frequency or route of administration is recommended anywhere in this document.

Important note on research references

Peptide research is not one-size-fits-all. Each compound is explored in multiple research contexts, for different objectives, using different experimental designs, timeframes and reference amounts. There is no universal protocol.

  • Reference amounts and schedules vary widely across the literature
  • Research intent, experimental design and model response all matter
  • The reference figures below are for calculation and consistency only — they are conversions, not recommendations

Researchers are strongly encouraged to work from primary literature and to conduct their own independent review of the published evidence for any compound. GANDER does not design protocols, does not advise on use, and does not select compounds on a customer's behalf.


HOW TO USE OUR RECONSTITUTION CALCULATOR

Peptides are best understood as signalling molecules — they influence pathways and environments, not outcomes in isolation.

Our calculator converts vial content + diluent volume into a concentration, and translates a target reference amount into a measurable volume (mL, or units on a graduated syringe scale). This is for calculation consistency only.

What you'll need

  • Vial content (e.g. 5mg, 10mg, 20mg, 100mg, 500mg)
  • Diluent volume (mL of bacteriostatic water added)
  • Target reference amount (mg or mcg) taken from the literature you are working from
  • Syringe scale: a 1.0mL graduated syringe is marked in 100 units, so 1 unit = 0.01mL

Step-by-step

  1. Enter vial content (e.g. 10mg).
  2. Enter diluent volume in mL (e.g. 2mL).
  3. The calculator outputs concentration (e.g. 5mg/mL, or 5000mcg/mL).
  4. Enter target reference amount (e.g. 500mcg).
  5. The calculator outputs mL to withdraw (e.g. 0.10mL) and the equivalent units on a 100-unit graduated syringe (e.g. 10 units).
  6. Sanity check: doubling the diluent volume halves the concentration; doubling the target amount doubles the volume required.

Common calculation mistakes

  • mg vs mcg confusion (1mg = 1000mcg)
  • Entering diluent volume in the wrong unit (mL only)
  • Assuming "units" equal mg — units are a volumetric scale; the mass depends entirely on concentration

Tip: if the literature is written in mcg but your vial is labelled in mg, convert first (mg × 1000 = mcg), or let the calculator handle the conversion.


QUICK REFERENCE (CALCULATION ONLY)

Concentration checks and calculator inputs.

  • BPC-157 (5mg): add 2ml → 2.5mg/ml (2500mcg/ml)
  • GHK-Cu (100mg): add 2ml → 50mg/ml
  • TB-500 (5mg): add 1ml → 5mg/ml
  • NAD+ (500mg): add 5ml → 100mg/ml
  • Semax (10mg): add 2ml → 5mg/ml (5000mcg/ml)
  • MOTS-c (10mg): add 1ml → 10mg/ml
  • Retatrutide (20mg): add 2ml → 10mg/ml
  • Ipamorelin (5mg): add 2ml → 2.5mg/ml (2500mcg/ml)
  • KPV (10mg): add 2ml → 5mg/ml (5000mcg/ml)

General handling principles
  • Use sterile technique
  • Allow lyophilised compounds to reconstitute naturally
  • Do not shake or agitate
  • Store reconstituted solutions refrigerated (not frozen unless specified)
  • Reference volumes are used for calculation consistency only

Peptides are best understood as signalling molecules — they influence pathways and environments, not outcomes in isolation.

BPC-157 — 5mg

Product Description
Synthetic research peptide supplied in lyophilised powder form.

Research Background (Educational Reference)
Research models explore signalling pathways involved in tissue integrity and inflammatory modulation, including cellular repair signalling, angiogenic pathways, gut–brain axis interactions and nitric oxide (NO) modulation.

Reconstitution
Add 2ml bacteriostatic water • Allow to dissolve naturally • Do not shake

Resulting Concentration
2.5mg per 1ml • 2500mcg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 0.25mg (250mcg) • 0.25ml (25 units) = 0.625mg (625mcg) • 0.40ml (40 units) = 1mg

Aliquots per vial
8 × 0.25ml aliquots

GHK-Cu — 100mg

Product Description
Copper-binding tripeptide supplied for laboratory research purposes.

Research Background (Educational Reference)
Studied for gene expression modulation and extracellular matrix regulation, including collagen and elastin signalling, cellular turnover pathways, anti-inflammatory cascades and oxidative stress modulation.

Reconstitution
Add 2ml bacteriostatic water • Allow to dissolve fully • Do not shake

Resulting Concentration
50mg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 5mg • 0.20ml (20 units) = 10mg • 0.50ml (50 units) = 25mg

Aliquots per vial
10 × 0.20ml aliquots

TB-500 — 5mg

Product Description
Thymosin Beta-4 fragment supplied as a lyophilised research compound.

Research Background (Educational Reference)
Studied for actin regulation, cellular migration and tissue repair signalling in experimental models.

Reconstitution
Add 1ml bacteriostatic water • Allow to dissolve naturally • Do not shake

Resulting Concentration
5mg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 0.5mg • 0.50ml (50 units) = 2.5mg • 1.00ml (100 units) = 5mg

Aliquots per vial
1 × 1.00ml, or 2 × 0.50ml aliquots

NAD+ — 500mg

Product Description
Coenzyme supplied in powder form for biochemical research.

Research Background (Educational Reference)
Studied for mitochondrial function, redox balance, ATP production and cellular longevity pathways, including PARP and sirtuin-related signalling.

Reconstitution
Add 5ml bacteriostatic water • Allow to dissolve fully • Do not shake

Resulting Concentration
100mg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 10mg • 0.25ml (25 units) = 25mg • 0.50ml (50 units) = 50mg

Aliquots per vial
10 × 0.50ml aliquots

Semax — 10mg

Product Description
Synthetic peptide supplied for experimental research use.

Research Background (Educational Reference)
Studied for neurotransmitter modulation and neuroplastic signalling, including BDNF-associated pathways, cognitive models, stress-response modulation and cerebral blood flow signalling.

Reconstitution
Add 2ml bacteriostatic water • Allow to dissolve naturally • Do not shake

Resulting Concentration
5mg per 1ml • 5000mcg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 0.5mg (500mcg) • 0.25ml (25 units) = 1.25mg (1250mcg) • 0.50ml (50 units) = 2.5mg

Aliquots per vial
8 × 0.25ml aliquots

MOTS-c — 10mg

Product Description
Mitochondrial-derived peptide supplied for laboratory research.

Research Background (Educational Reference)
Studied for metabolic regulation and stress-adaptation signalling, including AMPK activation, metabolic flexibility and mitochondrial–nuclear communication models.

Reconstitution
Add 1ml bacteriostatic water • Allow to dissolve fully • Do not shake

Resulting Concentration
10mg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 1mg • 0.25ml (25 units) = 2.5mg • 0.50ml (50 units) = 5mg

Aliquots per vial
2 × 0.50ml, or 4 × 0.25ml aliquots

Retatrutide — 20mg

Product Description
Multi-receptor agonist peptide supplied strictly for research use.

Research Background (Educational Reference)
Studied for interaction with GLP-1, GIP and glucagon receptors in metabolic signalling research — energy balance, glucose homeostasis, lipid metabolism and satiety signalling models.

Reconstitution
Add 2ml bacteriostatic water • Allow to dissolve naturally • Do not shake • Store refrigerated (do not freeze)

Resulting Concentration
10mg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 1mg • 0.20ml (20 units) = 2mg • 0.50ml (50 units) = 5mg

Aliquots per vial
20 × 0.10ml aliquots

Ipamorelin — 5mg

Product Description
Selective growth hormone secretagogue supplied in lyophilised form for research use.

Research Background (Educational Reference)
Studied for selective interaction with the ghrelin (GHS-R1a) receptor and endocrine signalling models, frequently referenced for receptor selectivity in experimental work.

Reconstitution
Add 2ml bacteriostatic water • Allow to dissolve naturally • Do not shake

Resulting Concentration
2.5mg per 1ml • 2500mcg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 0.25mg (250mcg) • 0.20ml (20 units) = 0.5mg (500mcg) • 0.40ml (40 units) = 1mg

Aliquots per vial
20 × 0.10ml aliquots

KPV — 10mg

Product Description
Tripeptide supplied in lyophilised form for laboratory research.

Research Background (Educational Reference)
α-MSH fragment studied for immune modulation and inflammatory signalling regulation in research models, including NF-κB and cytokine-related pathways.

Reconstitution
Add 2ml bacteriostatic water • Allow to dissolve fully • Do not shake

Resulting Concentration
5mg per 1ml • 5000mcg per 1ml

Volume Conversions (calculation only)
0.10ml (10 units) = 0.5mg (500mcg) • 0.20ml (20 units) = 1mg • 0.50ml (50 units) = 2.5mg

Aliquots per vial
20 × 0.10ml aliquots


Final disclaimer
All compounds listed are supplied for in-vitro research and laboratory purposes only. Every figure in this guide is a unit conversion provided for arithmetic consistency — no amount, frequency, schedule or route of administration is recommended. Nothing in this document should be interpreted as approval or instruction for use in humans or animals, or as medical, diagnostic or treatment advice. Not for sale to minors.